Tracing life at the dry limit using phospholipid fatty acids – does sampling matter?

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Title:Main Title: Tracing life at the dry limit using phospholipid fatty acids – does sampling matter?
Description:Abstract: Phospholipid fatty acids (PLFAs) have been used to trace bacterial life in extremely carbon-poor soils of the hyperarid Atacama Desert. However, the low abundances of bacteria and, thus, PLFAs increases the risk of contamination by exogenous PLFAs. Here, we assess whether field sampling strategies (super-clean, clean, and regular sampling protocols) have an effect on PLFA diversity and abundance in hyperarid Atacama soils or whether laboratory processing or true environmental heterogeneity control PLFA inventories. Our results show no exogenous PLFA contribution during sample processing in the lab and statistical analyses (ANOVA, Kruskal-Wallis) reveal that PLFA abundances do not differ significantly between replicate samples (n=3) taken with the three different sampling strategies. Rather than sampling strategy, our results show that PLFA abundances in the investigated soil replicates rather reflect true environmental heterogeneity of primarily bacterial biomass (in the absence of indigenous fungi), potentially related to small-scale physicochemical differences.
Responsible Party
Creators:Stephanie Kusch (Author), Andrea Jaeschke (Author), Ramona Mörchen (Author), Janet Rethemeyer (Author)
Funding Reference:Deutsche Forschungsgemeinschaft (DFG): CRC 1211: Earth - Evolution at the Dry Limit
Publisher:Elsevier Science, Amsterdam, The Netherlands
Publication Year:2019
Topic
CRC1211 Topic:Biology
Related Subprojects:D4, B5
Subjects:Keywords: Biogeochemistry of Soils, Organic Geochemistry, Soil Sciences
Geogr. Information Topic:Biota
File Details
Filename:Kusch2020.pdf
Data Type:Text - Article
File Size:1.2 MB
Date:Available: 08.11.2019
Mime Type:application/pdf
Data Format:PDF
Language:English
Status:Completed
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Download Permission:Only Project Members
General Access and Use Conditions:According to the CRC1211DB data policy agreement.
Access Limitations:According to the CRC1211DB data policy agreement.
Licence:None
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Specific Information - Publication
Publication Status:In Print
Review Status:Peer reviewed
Publication Type:Article
Article Type:Journal
Source:Soil Biology and Biochemistry
Volume:141
Number of Pages:5 (1 - 5)
Metadata Details
Metadata Creator:Andrea Jaeschke
Metadata Created:22.11.2019
Metadata Last Updated:22.11.2019
Subproject:D4
Funding Phase:1
Metadata Language:English
Metadata Version:V50
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