Comparative Studies of Terrestrial Gregarines Infecting Insects

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Title:Main Title: Comparative Studies of Terrestrial Gregarines Infecting Insects
Description:Abstract: Gregarines constitute a large group of early branching endobiotic apicomplexans (Alveolata, Eukaryota), inhabiting different compartments of invertebrates, like intestines, coeloms and reproductive organs. Despite a high number of researches on gregarines, their function and phylogeny, is not clarified. The assumption is, that gregarines co-evolved with their hosts and thus, are host-specific. To get an idea regarding their evolutionary origin, morphological characterisations and rDNA-based molecular analyses are used. Since molecular analyses of gregarines turned out to be more difficult than in previous studies initially expected, the hypothesis was, that most gregarine cells did not contain DNA after isolation. The aim was, to establish a universal processing protocol for gregarine’s single cell sequencing. In this study, we established a lysis protocol for gregarines, including the proof and extraction of DNA from the protoplast, for molecular analysis of the rDNA gene, using chosen primer. The LSU rDNA sequences of an undescribed Gregarina sp., from Tenebrio molitor larvae and of Atacamagregarina sp., from Psectrascelis sp., were obtained for the first time. In addition, the previously described SSU rDNA of Atacamagregarina psectrascelii and Atacamagregarina paposa, could be amplified again and confirmed. A probably undescribed gregarine species, from T. molitor larvae was found and described, differing significantly from all other gregarine species of T. molitor larvae. The phylogenetic analysis of the LSU rDNA showed that Gregarina sp. from T. molitor larvae clusters with Gregarina niphandrodes from T. molitor adult, whereas Atacamagregarina sp. from Psectrascelis sp. clusters with other terrestrial gregarines (Neogregarinorida sp. and Ascogregarina taiwanensis). This study is a stepping stone, as it may allow to study inter- and intraspecific relationships of gregarines, co occurring in endemic darkling beetles from the Atacama Desert, analysing conservatives and variables regions of the primary rDNA gene (SSU, ITS1, 5.8S, ITS2, LSU).
Responsible Party
Creator:Yana Feuling (Author)
Publisher:CRC1211 Database (CRC1211DB)
Publication Year:2019
Topic
CRC1211 Topic:Biology
Related Subproject:B3
Subject:Keyword: Biogeography
Geogr. Information Topic:Biota
File Details
Filename:FeulingBA.pdf
Data Type:Text - Text
File Size:513 KB
Date:Available: 13.08.2018
Mime Type:application/pdf
Data Format:PDF
Language:English
Status:Completed
Constraints
Download Permission:Only Own Subproject
General Access and Use Conditions:According to the CRC1211DB data policy agreement.
Access Limitations:According to the CRC1211DB data policy agreement.
Licence:None
Geographic
Specific Information - Report
Report Date:13th of August, 2018
Report Type:Bachelor Thesis
Report City:Cologne
Report Institution:University of Cologne
Metadata Details
Metadata Creator:Frank Nitsche
Metadata Created:24.10.2019
Metadata Last Updated:24.10.2019
Subproject:B3
Funding Phase:1
Metadata Language:English
Metadata Version:V50
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