TESTING PROTISTS WITHIN EXTREME HABITATS IN THE CONTEXT TO GLOBAL DISTRIBUTION

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Title:Main Title: TESTING PROTISTS WITHIN EXTREME HABITATS IN THE CONTEXT TO GLOBAL DISTRIBUTION
Description:Abstract: This project focuses on assessing protist species richness within diverse, extreme habitats by means of cultivation, morphology, and DNA sequencing, while relating those findings to the context of further evaluating the possible global distribution of protists. This study is made up of two environmental segments. The first consists of a long term study that started in the year 2000 focusing on further investigating protist distribution via air settling on the Cologne Cathedral (Kölner Dom) in Germany. The second was the first of its kind; investigating the possible presence of heterotrophic protists within soils of the driest and oldest desert on Earth, the Atacama Desert. 20 deposition samples in 2013 and 12 in 2000, were taken from the Dom’s roof, whereas 29 soil samples in April, 2013, were taken from the Atacama. All samples were cultivated in freshwater medium and examined over a period of four weeks. The morpho-species of protists were determined. Single cells of isolated Protozoa were manually picked using a micromanipulator and sequenced in order to be classified into phylogenetic trees. Protozoa morpho-species from Dom 2013 samples were not significantly different from those that were stored for 13 years under dry conditions. 17 different protist taxa were observed from one Atacama Desert soil sample. Ciliophora was the most common group. The presence of vegetation within the positive sampling site represents an oasis where protists can successfullyobtain water. Additional temperature experiments were conducted on five selected ciliate morpho-species to test their tolerance to gradual warming conditions. The ciliate Cyrtolophosis sp. was able to survive up to 39˚C. Cysts from four of the ciliate morpho-species were able to withstand a 24-hour exposure to 50˚C and excyst within ten days after being reintroduced to room temperature. Morphological and phylogenetic results have helped prove that some protists exhibit cosmopolitan distributions, but they also help suggest that through speciation within certain extreme habitats, endemic species may be present.
Responsible Party
Creator:Glen Bornhoft (Author)
Publisher:CRC1211 Database (CRC1211DB)
Publication Year:2019
Topic
CRC1211 Topic:Biology
Related Subproject:B3
Subject:Keyword: Biodiversity
Geogr. Information Topic:Biota
File Details
Filename:Bornhoft.pdf
Data Type:Text - Text
File Size:31 KB
Date:Available: 10.12.2013
Mime Type:application/pdf
Data Format:PDF
Language:English
Status:Completed
Constraints
Download Permission:Only Own Subproject
General Access and Use Conditions:According to the CRC1211DB data policy agreement.
Access Limitations:According to the CRC1211DB data policy agreement.
Licence:None
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Specific Information - Report
Report Date:10th of December, 2013
Report Type:Bachelor Thesis
Report City:Cologne
Report Institution:University of Cologne
Metadata Details
Metadata Creator:Frank Nitsche
Metadata Created:25.10.2019
Metadata Last Updated:25.10.2019
Subproject:B3
Funding Phase:1
Metadata Language:English
Metadata Version:V50
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