Curated fasta sequences of 18S SSrRNA of nematodes isolated in the Atacama Desert during sampling in 2022

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Title:Main Title: Curated fasta sequences of 18S SSrRNA of nematodes isolated in the Atacama Desert during sampling in 2022
Description:Other: File containing 299 fasta sequences corresponding to SSrRNA sequences from desert nematodes. DNA was extracted using a modified version of the HotShot DNA extraction method (, primers and PCR conditions implanted have been previously published by other authors (, sequencing was performed using Sanger technology. Sequencing results are final for sampling campaigns conducted in 2022.
Responsible Party
Creators:Laura Villegas (Author), Laura Pettrich (Author), Oleksandr Holovachov (Author), Ann-Marie Waldvogel (Author), Philipp Schiffer (Author)
Publisher:CRC1211 Database (CRC1211DB)
Publication Year:2023
CRC1211 Topic:Biology
Related Subproject:B8
Subjects:Keywords: Biodiversity, Molecular Biology
Geogr. Information Topic:Biota
File Details
Data Type:Dataset - Coordinates of different nemat
File Size:257 KB
Date:Updated: 01.06.2023
Mime Type:text/plain
Data Format:OTHER
Download Permission:Only Project Members
General Access and Use Conditions:According to the CRC1211DB data policy agreement.
Access Limitations:According to the CRC1211DB data policy agreement.
Licence:[CRC1211DB] Data policy agreement
Specific Information - Data
Temporal Extent:02.03.2022 - 22.10.2022
Lineage:Sequence quality was assessed using Geneious PrimeĀ® (v. 2022.1.1). Only sequences with at least 20% high quality bases were kept for further processing. Sequences were trimmed at the ends and blasted against the curated 18S-NemaBase from (10.2478/jofnem-2023-0006) using the balstn function in Geneious Prime, best hits were assigned according to the lowest E-value and a high percentage of identity.
Subtype:Natural Science Data
Metadata Details
Metadata Creator:Laura Villegas
Metadata Created:31.08.2023
Metadata Last Updated:31.08.2023
Funding Phase:2
Metadata Language:English
Metadata Version:V50
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