Curated fasta sequences of 18S SSrRNA of nematodes isolated in the Atacama Desert during sampling in 2022
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Identification
| Title: | Main Title: Curated fasta sequences of 18S SSrRNA of nematodes isolated in the Atacama Desert during sampling in 2022 |
| Description: | Other: File containing 299 fasta sequences corresponding to SSrRNA sequences from desert nematodes. DNA was extracted using a modified version of the HotShot DNA extraction method (https://doi.org/10.4319/lom.2008.6.218), primers and PCR conditions implanted have been previously published by other authors (https://doi.org/10.1111/j.1471-8286.2005.01009.x), sequencing was performed using Sanger technology. Sequencing results are final for sampling campaigns conducted in 2022. |
Responsible Party
| Creators: | Laura Villegas (Author), Laura Pettrich (Author), Oleksandr Holovachov (Author), Ann-Marie Waldvogel (Author), Philipp Schiffer (Author) |
| Publisher: | CRC1211 Database (CRC1211DB) |
| Publication Year: | 2023 |
Topic
| CRC1211 Topic: | Biology |
| Related Subproject: | B8 |
| Subjects: | Keywords: Biodiversity, Molecular Biology |
| Geogr. Information Topic: | Biota |
File Details
| Filename: | NematodeSequences18SB08.txt |
| Data Type: | Dataset - Coordinates of different nemat |
| File Size: | 257 KB |
| Date: | Updated: 01.06.2023 |
| Mime Type: | text/plain |
| Data Format: | OTHER |
| Language: | English |
| Status: | Completed |
Constraints
| Download Permission: | Only Project Members |
| General Access and Use Conditions: | According to the CRC1211DB data policy agreement. |
| Access Limitations: | According to the CRC1211DB data policy agreement. |
| Licence: | [CRC1211DB] Data policy agreement |
Geographic
Specific Information - Data
| Temporal Extent: | 02.03.2022 - 22.10.2022 |
| Lineage: | Sequence quality was assessed using Geneious Prime® (v. 2022.1.1). Only sequences with at least 20% high quality bases were kept for further processing. Sequences were trimmed at the ends and blasted against the curated 18S-NemaBase from (10.2478/jofnem-2023-0006) using the balstn function in Geneious Prime, best hits were assigned according to the lowest E-value and a high percentage of identity. |
| Subtype: | Natural Science Data |
Metadata Details
| Metadata Creator: | Laura Villegas |
| Metadata Created: | 31.08.2023 |
| Metadata Last Updated: | 31.08.2023 |
| Subproject: | B8 |
| Funding Phase: | 2 |
| Metadata Language: | English |
| Metadata Version: | V50 |
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Dataset Statistics
| Page Visits: | 28 |
| Metadata Downloads: | 0 |
| Dataset Downloads: | 0 |
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