Curated fasta sequences of 18S SSrRNA of nematodes isolated in the Atacama Desert during sampling in 2022

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Title:Main Title: Curated fasta sequences of 18S SSrRNA of nematodes isolated in the Atacama Desert during sampling in 2022
Description:Other: File containing 299 fasta sequences corresponding to SSrRNA sequences from desert nematodes. DNA was extracted using a modified version of the HotShot DNA extraction method (https://doi.org/10.4319/lom.2008.6.218), primers and PCR conditions implanted have been previously published by other authors (https://doi.org/10.1111/j.1471-8286.2005.01009.x), sequencing was performed using Sanger technology. Sequencing results are final for sampling campaigns conducted in 2022.
Responsible Party
Creators:Laura Villegas (Author), Laura Pettrich (Author), Oleksandr Holovachov (Author), Ann-Marie Waldvogel (Author), Philipp Schiffer (Author)
Publisher:CRC1211 Database (CRC1211DB)
Publication Year:2023
Topic
CRC1211 Topic:Biology
Related Subproject:B8
Subjects:Keywords: Biodiversity, Molecular Biology
Geogr. Information Topic:Biota
File Details
Filename:NematodeSequences18SB08.txt
Data Type:Dataset - Coordinates of different nemat
File Size:257 KB
Date:Updated: 01.06.2023
Mime Type:text/plain
Data Format:OTHER
Language:English
Status:Completed
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Download Permission:Only Project Members
General Access and Use Conditions:According to the CRC1211DB data policy agreement.
Access Limitations:According to the CRC1211DB data policy agreement.
Licence:[CRC1211DB] Data policy agreement
Geographic
Specific Information - Data
Temporal Extent:02.03.2022 - 22.10.2022
Lineage:Sequence quality was assessed using Geneious PrimeĀ® (v. 2022.1.1). Only sequences with at least 20% high quality bases were kept for further processing. Sequences were trimmed at the ends and blasted against the curated 18S-NemaBase from (10.2478/jofnem-2023-0006) using the balstn function in Geneious Prime, best hits were assigned according to the lowest E-value and a high percentage of identity.
Subtype:Natural Science Data
Metadata Details
Metadata Creator:Laura Villegas
Metadata Created:31.08.2023
Metadata Last Updated:31.08.2023
Subproject:B8
Funding Phase:2
Metadata Language:English
Metadata Version:V50
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