Protist communities of microbial mats from extreme environments of five saline Andean lagoons at high elevation in the Atacama Desert

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Title:Main Title: Protist communities of microbial mats from extreme environments of five saline Andean lagoons at high elevation in the Atacama Desert
Description:Abstract: Heterotrophic protists colonizing microbial mats have received little attention over the last years despite their important role in the microbial foodwebs, as those found in the remote Andean lagoons. We sampled these mats from different isolated aquatic systems distributed across the Altiplano, where extreme radiation and wide temperature changes prevail, potentially structuring the microbial communities. Here, we present the results of the metabarcoding from DNA and cDNA of protists (V9) in microbial mats across high-altitude lagoons of different salinity (4.3-34 PSU), including insights into their vertical stratification. We assessed the DNA-based protists detected across all the study sites. However, for the cDNA-based analysis, amplification was achieved primarily in three study sites with lower salinity. Sequence variants classified as the amoeboid rhizarian Rhogostoma and as the ciliate Euplotes were found to be common members of the heterotrophic protist community in both the DNA and cDNA- based analyses of the studied lagoons. They were accompanied by diatoms and kinetoplastids. As detected through DNA, different ASVs classified as Rhogostoma and Neobodo are the shared species between the most saline lagoons. Furthermore, our analyses point to salinity of the water column as a main driver influencing the structure of the protist communities at the five studied microbial mats. Interestingly, the sequence of ASVs classified as Rhogostoma are highly similar to the sequence from R. olyaorum, a species recently isolated from the Atacama Desert. Our results provide a first snapshot of the unculturable and active protist diversity thriving five athalossohaline lagoons from the Andean plateau.
Responsible Party
Creators:Eduardo Acosta (Author), Frank Nitsche (Author), Christina Dorador (Author), Hartmut Arndt (Principal Investigator)
Funding Reference:Deutsche Forschungsgemeinschaft (DFG): CRC 1211: Earth - Evolution at the Dry Limit
Publisher:Frontiers
Publication Year:2023
Topic
CRC1211 Topic:Biology
Related Subprojects:B2, B3
Subject:Keyword: Biodiversity
Geogr. Information Topic:Biota
File Details
Filename:Acosta_et_al_subm_version.pdf
Data Type:Data Paper - NGS data
File Size:23.7 MB
Date:Submitted: 16.12.2023 (pdf)
Mime Type:application/pdf
Data Format:PDF
Language:English
Status:Completed
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Download Permission:Only Project Members (Download Embargo will be lifted after project end)
General Access and Use Conditions:According to the CRC1211DB data policy agreement.
Access Limitations:According to the CRC1211DB data policy agreement.
Licence:[Creative Commons] Attribution 4.0 International (CC BY 4.0)
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Specific Information - Publication
Publication Status:Submitted
Review Status:Peer reviewed
Publication Type:Article
Article Type:Journal
Source:Frontiers of Microbiology
Number of Pages:25 (1 - 25)
Metadata Details
Metadata Creator:Frank Nitsche
Metadata Created:21.12.2023
Metadata Last Updated:21.12.2023
Subproject:B2
Funding Phase:2
Metadata Language:English
Metadata Version:V50
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