The Protistan Microbiome of Grassland Soil: Diversity in the Mesoscale

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Title:Main Title: The Protistan Microbiome of Grassland Soil: Diversity in the Mesoscale
Description:Abstract: Genomic data for less than one quarter of ∼1.8 million named species on earth exist in public databases like GenBank. Little information exists on the estimated one million small sized (1–100 m) heterotrophic nanoflagellates and ciliates and their taxa-area relationship. We analyzed environmental DNA from 150 geo-referenced grassland plots representing topographical and land-use ranges typical for Central Europe. High through-put barcoding allowed the identification of operational taxonomic units (OTUs) at species level, with high pairwise identity to reference sequences (≥99.7%), but also the identification of sequences at the genus (≥97%) and class (≥80%) taxonomic level. Species richness analyses revealed, on average, 100 genus level OTUs (332 unique individual read (UIR) and 56 class level OTUs per gram of soil sample in the mesoscale (1–1 000 km). Database shortfalls were highlighted by increased uncertain taxonomic lineages at lower resolution (≥80% sequence identity). No single barcode occurred ubiquitously across all sites. Taxa-area relationships indicated that OTUs spread over the entire mesoscale were more similar than in the local scale and increased land-use (fertilization, mowing and grazing) promoted taxa-area separation. Only a small fraction of sequences strictly matched reference library sequences, suggesting a large protistan “dark matter” in soil which warrants further research.
Responsible Party
Creators:Paul Venter (Author), Frank Nitsche (Author), Anne Domonell (Author), Peter Heger (Author), Hartmut Arndt (Author)
Publisher:Esevier
Publication Year:2019
Topic
CRC1211 Topic:Biology
Related Subproject:B3
Subject:Keyword: Biogeography
File Details
Filename:Venter2017.pdf
Data Type:Text - Article
File Size:2.9 MB
Date:Available: 30.03.2017
Mime Type:application/pdf
Data Format:PDF
Language:English
Status:Completed
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Download Permission:Free
General Access and Use Conditions:According to the CRC1211DB data policy agreement.
Access Limitations:According to the CRC1211DB data policy agreement.
Licence:None
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Specific Information - Publication
Publication Status:Accepted
Review Status:Not peer reviewed
Publication Type:Article
Article Type:Journal
Source:Protist
Source Website:http://www.elsevier.de/protis
Volume:168
Number of Pages:109 (546 - 654)
Metadata Details
Metadata Creator:Frank Nitsche
Metadata Created:17.06.2019
Metadata Last Updated:17.06.2019
Subproject:B3
Funding Phase:1
Metadata Language:English
Metadata Version:V50
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